# Pressure-retained seawater (PRS) single-cell analysis # PI: Douglas Bartlett (UCSD) # Version: 13 March 2017 # Note: # These are the four types of deep-sea messure-retained seawn the prr sample obtained durinle obtained during the firhe first drop of the Leggo lander. # The cells were identified following cell sorting, MDA amplification, and 16S rRNA gene screening. ========================= SILVA_classification partial_16S_seq ------------------------- Bacteria;Bacteroidetes;Flavobacteriia;Flavobacteriales;Flavobacteriaceae;Aquibacter; TTGGGTTTAAAGGGTCCGTAGGTGGATAATTAAGTCAGAGGTGAAATCCTGCAGCTCAACTGTAGAATTGCCTTTGAAACTGGTTATCTTGAGTCATTATGAAGTGGTTAGAATGTGTAGTGTAGCGGTGAAATGCATAGATATTACACGGAATACCGATTGCGAAGGCAGATCACTAATAATGTACTGACACTGATGGACGAAAGCGTGGGTAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGGATACTAGCTGTTCGGATTTAGGTCTGAGTGGCTAAGCGAAAGTGATAAGTATCCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCANNNNAATTGACGGCTGTCTCTTATACACATCTCCGAGCCCCACGAGACAA Bacteria;Proteobacteria;Betaproteobacteria;Rhodocyclales;Rhodocyclaceae;Dechloromonas; GTGGGTTCNTAAGACAGAGGTGANTCCCCGGGCTCAACCTGGGNNNNGCCTTTGTGACTGCGAGGCTAGAGTACGGCAGAGGGGGGTGGAATTCCACGTGTANCATGAAATGCGTNNNATGGGNAGGAACACCGGTGGNGAAGGCCTTCCNCNGGGCCNATACTGACGCTCATGCNTNAAAGNGTGGGTATAAACAGGATTATATACCCTGNNANTCCACGNCCTAAACGATGTCNNCTATCTNNNGNGTGGTTNACACCCTTTANCACC Bacteria;Planctomycetes;Phycisphaerae;Phycisphaerales;Phycisphaeraceae;Urania-1B-19; TACGCGGAATTACTGGGNTTAAAGCGTACGTAGGCGGATCTACAGGTATCCTGTGAAAGCCCACGGCTCAACCGTGGAATTGCAGGGTAAACCGTAGATCTTGAGGTGGCTAGGGGCTCTCGGAACGGTAGGTGGAGTGGTGAAATGCGTTGATATCTACCGGAACGCCAAAGGGGAAGCCAGGGAGCTGGGGCCATTCTGACGCTGAGGTACGAAAGCGTGGGTAGCGAACGGGATTAGATACCCCGGTAGTCCACGCCGTAAACGATGTGCACTAGGTCGAGGGGGTTTGACGCCGCTCTCGGCTGAAGCGAAAGTGTTAAGTGCACCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGNAATTGACGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACA Bacteria;Marinimicrobia (SAR406 clade); AAAGAGTTCGTAGGCTGNTTGGTCAGTCGAAATTGAAATCCCACGGCTTAACCGTGGACCCGGTTTTGATACTGCCAATCTTGAGTGAGGTAGAGGTAAGCGGAATTCCTGGTGTAGCGGTGAAATGCGTAGATATCAGGAGGAACACCAAAGGCGAAGGCAGCTTACTGGACCTATACTGACGCTGAGGAACGAAAGCGTGGGGAGCAAACAGGATTAGAGACCCTGGTAGTCCACGCCGTAAACGATGAGTACTTGGTGGTGGGGGATTCGACCCCTTCACTGTCGGAGCTAACGCGTTAAGTACTCCGCCTGGGAACTACGACCGCAAGGTTGAAACTCAAAGGAATTGACGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACA